FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84797474
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC24406822.878248472354259TruSeq Adapter, Index 9 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4563020.5381080101513401No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2659390.31361665325077964No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1882630.2220148680372248No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1327070.15649876551747283No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA973570.11481120298465494No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)