FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83660010
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC20086972.401024097415241TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4880670.5833934277559851No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3162000.37795835788209925No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2015850.24095741800652426No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1639370.19595622807121346No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1011860.12094906515072136No Hit
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA960510.11481112660636786No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA912930.10912382152476435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)