FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65251083
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC9985401.5303041023855497TruSeq Adapter, Index 23 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2480490.3801454145979462No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1828880.28028347054408276No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT873570.1338782376991352No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA785150.12032750475574482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)