FastQCFastQC Report
Sat 13 May 2023
EGAF00004457686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71132548
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC16256852.285430573919551TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3505170.49276598386437664No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGACCTCGTATGC2607260.36653544309983105TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2360930.33190572619442793No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1342830.18877856027313966No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1127290.1584773822526363No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)