FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78397193
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC13913161.7747012957466475TruSeq Adapter, Index 12 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3064380.39087879077507276No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2446170.31202265111711336No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1131330.14430746264091368No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1058080.1349640158672518No Hit
TGGTTGGGAATGTGATGGTGAGATGTGATATTGTGAAGGATGGTAGTATG936470.1194519808891627No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)