FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63796963
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC62597579.811998417542227TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACGCGTCTGAACTCCAGTCACTCATTCATCTCGTATGC2307380.3616755236452243TruSeq Adapter, Index 3 (97% over 34bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1154210.18091927040476832No Hit
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCATTCATCTCGTATGC1074840.16847823931681513TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCATTCATCTCGTATGC1068260.16744684225799275TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCATTCATCTCGTATGC1060540.1662367533075203TruSeq Adapter, Index 3 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCT1054780.16533388901286727RNA PCR Primer, Index 45 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTCATTCATCTCGTATGC1022940.16034305582853528TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCACCTCGTATGC1011360.1585279224028266TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAGCTCGTATGC998670.15653879950366917TruSeq Adapter, Index 3 (97% over 36bp)
CGGGAGGTGAGGGGCGTTTTTGTTCGGTCGTTTTTATTGGGAAGTGAGGA983210.15411548665725672No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTCATTCATCTCGTATGC934980.14655556566227143TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCATTCATCTCGTATGC918120.14391280663313077TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTCATTCATCTCGTATGC915180.14345196964940166TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAACTCGTATGC911590.14288924693797728TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATTCATCTCGTATGC909130.14250364864546922TruSeq Adapter, Index 3 (97% over 36bp)
CGGAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTC867630.1359986368003129RNA PCR Primer, Index 45 (100% over 34bp)
GATCGGAAGAGCACCGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC836670.1311457412165529RNA PCR Primer, Index 45 (100% over 31bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC830180.13012845141233448TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC806760.12645743026983902RNA PCR Primer, Index 45 (100% over 31bp)
GATCGGAAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCG791640.12408741149637482RNA PCR Primer, Index 45 (100% over 32bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC710650.11139244982555047RNA PCR Primer, Index 45 (100% over 31bp)
GATCGGAAGAGCACACTCTGAACTCCAGTCACTCATTCATCTCGTATGCC689140.10802081597520558RNA PCR Primer, Index 45 (100% over 31bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA668320.10475733774349101No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC664650.10418207525019647TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGACACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC649650.10183086614953755RNA PCR Primer, Index 45 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)