FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83900939
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC60717877.236852259782217TruSeq Adapter, Index 2 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2949100.35149785391555627No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2280760.27183962744445567No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACACCTCGTATGC2025300.2413918156505972TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAACTCGTATGC1669220.19895128944861987TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACAGCTCGTATGC1262860.1505179816879046TruSeq Adapter, Index 2 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1183160.14101868395060513No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTATACATCTCGTATGC1152680.13738582830401935TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTATACATCTCGTATGC996760.11880200768670778TruSeq Adapter, Index 12 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA984190.11730381229702327No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTATACATCTCGTATGC980700.11688784555796211TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTATACATCTCGTATGC894110.10656734127850465TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTATACATCTCGTATGC885290.10551610155400049TruSeq Adapter, Index 12 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATACATCTCGTATGC882570.10519190971152301TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTATACATCTCGTATGC872810.10402863310028032TruSeq Adapter, Index 12 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)