FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75140121
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC16256832.1635352437082185TruSeq Adapter, Index 12 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA5924310.7884349826905388No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3685660.4905049327775237No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2226130.2962638295458694No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1604770.21357032416809657No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1381360.18383787271250202No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC1023350.1361922214631515No Hit
CGGGATGGTTTCGATTTTTTGATTTCGTGATTCGTTCGTTTCGGTTTTTT994240.13231812602484364No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT897630.1194608137508855No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT840730.11188829467016696No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA795130.1058196326300832No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)