FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74703492
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC69101199.250061563387158TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1978870.2648965860926555No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCC1553650.20797555220042457TruSeq Adapter, Index 3 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGT1460820.19554909160069783TruSeq Adapter, Index 3 (97% over 34bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGTCT1440130.19277947542264826RNA PCR Primer, Index 38 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1406130.18822814869216556No Hit
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTAGCTATCTCGTATGC1194040.1598372402725163TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTAGCTATCTCGTATGC1190760.15939817110557564TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGCTATCTCGTATGC1146180.15343057858660744TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC1139770.15257251963536056TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTAGCTATCTCGTATGC1126410.15078411595538266TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAGCTCGTATGC1109980.14858475424415232TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTAGCTATCTCGTATGC1101120.1473987320432089TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTAGCTATCTCGTATGC1097140.14686595909064062TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTAGCTATCTCGTATGC1029860.13785968666632079TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC966750.1294116210792395TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGTTATCTCGTATGC931640.12471170691726165TruSeq Adapter, Index 12 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAACTCGTATGC852280.11408837487811145TruSeq Adapter, Index 3 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT803550.10756525277292259No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)