FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77822645
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4292300.5515489739522474No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC3322990.42699525311687875TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3176650.40819095778613024No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1469510.18882807182922143No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1392730.17896204889977718No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA812190.1043642245775635No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT778790.1000724146551431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)