FastQCFastQC Report
Sat 13 May 2023
EGAF00004457695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75259863
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCCGAATCTCGTATGC7785821.0345248701821315TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3323700.4416298233229577No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2517330.3344850627750944No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1126610.1496959939988198No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1066210.14167046782957868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)