FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64032365
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC59296579.260406046223656TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATGC1613250.25194290418603155TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAACTCGTATGC1586060.24769661404822388TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGACCTCGTATGC1286560.20092339241257134TruSeq Adapter, Index 10 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC1053610.16454335241248702TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC1036300.16184003199007252TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCGAAGATCTCGTATGC948640.14815008004155397TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCGAAGATCTCGTATGC937710.14644313075114435TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCGAAGATCTCGTATGC923490.14422237879234978TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAAGATCTCGTATGC888420.1387454609867994TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTCGAAGATCTCGTATGC854320.13342002907435951TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTCGAAGATCTCGTATGC824060.12869429389340842TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTCGAAGATCTCGTATGC807050.1260378247781415TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC771140.12042972331257795TruSeq Adapter, Index 3 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGCC760190.11871965060169182TruSeq Adapter, Index 10 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)