FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70903617
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGATCTCGTATGC22163543.1258687409416646TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2188180.30861331094011746No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1910960.26951516450846225No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAACTCGTATGC1229820.17344954348379715TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGAGCTCGTATGC1177490.16606910194722505TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGAAGACCTCGTATGC1064040.15006850778853778TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)