FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72069537
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC49435906.859472400939665TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAGCTCGTATGC1313900.18231003759605116TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAACTCGTATGC1304470.1810015790721675TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGACCTCGTATGC1004050.13931683784786905TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAAAAGATCTCGTATGC812330.11271475214278122TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAAAAGATCTCGTATGC774380.10744900442471277TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC764140.10602815444755806TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAAAAGATCTCGTATGC723780.10042800746728815TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)