FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84026421
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTTTATCTCGTATGC15120171.799454245468815TruSeq Adapter, Index 9 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2453570.29199982229399013No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1790890.21313415217339796No Hit
TGGGTTTATGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1108040.13186804659929524No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA947780.11279547417591426No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT862150.10260463194070826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)