FastQCFastQC Report
Sat 13 May 2023
EGAF00004457704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90527706
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC65513757.236872875139463TruSeq Adapter, Index 22 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2012300.222285539854506No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGTATCTCGTATGC1727390.19081340689225018TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAACTCGTATGC1694010.18712613793615845TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCAGCTCGTATGC1646990.1819321479327003TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCACCTCGTATGC1630680.18013048955421448TruSeq Adapter, Index 22 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1603990.17718222087721963No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1154530.12753333217125815TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGGTAGCATCTCGTATGC1028940.11366023126665775TruSeq Adapter, Index 11 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1011900.11177793459164866TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGGTAGCATCTCGTATGC1011750.11176136507866444TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGGTAGCATCTCGTATGC965320.10663254849294425TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGGTAGCATCTCGTATGC947960.10471490352356878TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGGTAGCATCTCGTATGC938980.10372294201291259TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGGTAGCATCTCGTATGC935810.10337277297184577TruSeq Adapter, Index 11 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)