FastQCFastQC Report
Sat 13 May 2023
EGAF00004457705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68861190
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC17233832.502691283726TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAACCTCGTATGC8533881.2392873257055244TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAACTCGTATGC1573410.22849009725216773TruSeq Adapter, Index 23 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1483310.21540580405305224No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAACCCCGTATGC1251300.18171338601612896TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAACCCCGTATCC1234210.17923158167902706TruSeq Adapter, Index 23 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1122550.16301635217166593No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAACCTCGTATCC1095350.15906637686627256TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)