FastQCFastQC Report
Sat 13 May 2023
EGAF00004457709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89011735
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA7604520.8543278029576662No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA5088240.5716369869658198No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2521800.2833109589426608No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA2398170.26942177904969494No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC2156470.24226805600407633TruSeq Adapter, Index 7 (97% over 35bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1480930.16637469205605307No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1373050.1542549417781824No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT1274530.14318673824299685No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1060660.11915956924106694No Hit
CGGGTTTAAGCGATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA982610.11039106248181771No Hit
CGGGTTTAAGCGATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA977890.10986079532097649No Hit
CGGGCGCGGTGGTTTACGTTTGTAATTTTAGTATTTTGGGAGGTCGAGGC971490.1091417890011918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)