FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004457710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79482609
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGC26692793.3583182957670656TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA7920230.9964733291530479No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA5344310.6723873394744755No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2740720.3448200851081776No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA2421650.304676712361065No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1450350.18247387928597059No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1297800.16328100150814123No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1149210.14458634592631453No Hit
CGGGTTTAAGCGATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA868410.1092578629370357No Hit
CGGGTTTAAGCGATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA825820.10389945805629001No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)