FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68882841
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC1065124815.462846545484382TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGACCTCGTATGC2915280.42322296201458937TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAACTCGTATGC2770560.40221337560685105TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAGCTCGTATGC2361200.34278493246235303TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTGATCTCGTATGC1924590.2794004968523293TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC1872440.2718296709045436TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGGCGATCTCGTATGC1715560.24905476822595052TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCGATCTCGTATGC1710300.24829115279957747TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGGCGATCTCGTATGC1656870.2405345040864386TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGGCGATCTCGTATGC1655010.24026448038053486TruSeq Adapter, Index 7 (97% over 36bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC1620340.23523129657210276TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGGCGATCTCGTATGC1594690.23150758256326856TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGGCGATCTCGTATGC1574130.22852280439478392TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGGCGATCTCGTATGC1552910.22544221136291404TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC1325920.1924891570601741TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1224200.1777220541760175No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA924350.13419161965169235No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC768780.11160689495951538TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC749930.10887036439161968TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)