FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76443445
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC57557857.529468354023029TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAACCTCGTATGC3788680.4956186890844597TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2025670.2649893656676514No Hit
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC1943000.25417483474220715TruSeq Adapter, Index 23 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1584390.2072630295508006No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAACTCGTATGC1560090.20408420892072562TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCAACAATCTCGTATGC1078080.14102975081774508TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCAACAATCTCGTATGC998360.13060112610047858TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAGCTCGTATGC958630.12540381977813794TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCAACAATCTCGTATGC955930.1250506174859074TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCCAACAATCTCGTATGC916080.11983761328391204TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCCAACAATCTCGTATGC897030.11734557488872982TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCCAACAATCTCGTATGC860450.11256033790732482TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCCAACAATCTCGTATGC856100.11199128976984225TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAACAATCTCGTATGC824800.10789675949324365TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACAATCTCGTATGC806610.10551722256892007TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC773940.1012434748329304TruSeq Adapter, Index 23 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)