FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004457714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74984282
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC12827081.7106358369878103TruSeq Adapter, Index 22 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA5945030.7928368241226875No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3888160.5185300033945781No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT2217140.29568063344261936No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1720030.22938540639757007No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1232070.16431043508558235No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA987650.13171427046537568No Hit
CGGGCGTGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT932850.12440607219523687No Hit
CGGGTTTAAGCGATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA756920.10094382179988068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)