FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67825746
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGATCTCGTATGC30226944.456558428417432TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3308440.4877852725718638No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGACCTCGTATGC3025680.4460960886445687TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2239960.33025217297278237No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAACTCGTATGC1795110.26466498429667107TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACCGGAGCTCGTATGC1374250.2026148005802988TruSeq Adapter, Index 7 (97% over 35bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1158480.1708023970720499No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA964390.14218641988840047No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCACCGGATCTCGTATGC746240.11002311718031085TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACCGGATCTCGTATGC708470.10445443534082176TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCACCGGATCTCGTATGC692990.10217211617547117TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCACCGGATCTCGTATGC687780.1014039712884249TruSeq Adapter, Index 7 (97% over 35bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT679680.10020973451585774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)