FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71874001
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC40507455.6358974645087585TruSeq Adapter, Index 23 (97% over 38bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3883400.54030664022725No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2502920.3482371880201855No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1414620.19681943127112123No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1068700.1486907623244739No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT871360.12123438070464451No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAAACTCGTATGC787080.10950830467890608TruSeq Adapter, Index 23 (97% over 38bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAACTAATCTCGTATGC757270.10536076877089395TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGCC756610.10526894140761693TruSeq Adapter, Index 23 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)