FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64878208
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC18008792.7757841276997044TruSeq Adapter, Index 12 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1963690.3026732797551992No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1481980.2284249281361162No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCACCTCGTATGC775720.11956557123156053TruSeq Adapter, Index 12 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT677250.10438790171269836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)