FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68440999
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC53445997.809060472656164TruSeq Adapter, Index 7 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3867140.5650326641199378No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2694170.3936485497530508No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAACTCGTATGC2078190.30364694121428587TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAGCTCGTATGC1641950.23990736897338394TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGACCTCGTATGC1560110.22794962417190898TruSeq Adapter, Index 7 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1397300.20416125135753788No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1115820.1630338563585257No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC1024400.14967636577017235TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAAAAGATCTCGTATGC855250.12496164762294017TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAAAAGATCTCGTATGC834850.12198097809764583TruSeq Adapter, Index 7 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC827030.12083838811294965TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAAAAGATCTCGTATGC782180.11428529849483932TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAAAAGATCTCGTATGC763070.1114931124836445TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAAAAGATCTCGTATGC758300.11079616181523008TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAAAGATCTCGTATGC756900.11059160606349419TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAAAAGATCTCGTATGC753060.1100305388587329TruSeq Adapter, Index 7 (97% over 35bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT720850.10532429545629513No Hit
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC696800.1018103198639751TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)