FastQCFastQC Report
Sun 14 May 2023
EGAF00004457732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72974362
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2802250.38400472757815957No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2227110.3051907463062164No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCATCTCGTATGC1371210.18790297885714985TruSeq Adapter, Index 1 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1069710.1465870986306122No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGAGCACCTCGTATGC1013810.13892687407119778TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA849930.11646967191025254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)