FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72631868
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC16579142.282626133200925TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2719500.37442242295076317No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGACCTCGTATGC2625920.36153827132740135TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2220100.3056647255719762No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT970730.13365069999301135No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAACTCGTATGC952700.13116831856782205TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA821740.11313766568691308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)