FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75929700
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC23555643.1022959395335423TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAACCTCGTATGC3599920.47411223803070474TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2884230.3798553135334395No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2356120.3103028195817974No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAACTCGTATGC1156760.15234618337751893TruSeq Adapter, Index 1 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1098860.14472070876086696No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT946760.12468902155546513No Hit
GAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCT821170.10814872177817111RNA PCR Primer, Index 40 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)