FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71323517
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC1022369914.334260886209524TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCAGCTCGTATGC2007540.28146957475470535TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCATGGCATCTCGTATGC1827910.25628433325855204TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1745020.24466264051448838No Hit
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCATGGCATCTCGTATGC1676620.2350725357528289TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCATGGCATCTCGTATGC1643100.23037282359477587TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCAACTCGTATGC1526700.2140528207547589TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCATGGCATCTCGTATGC1520430.21317372781827346TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCATGGCATCTCGTATGC1459270.20459871601676624TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCATGGCATCTCGTATGC1453880.2038430045450507TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCATGGCATCTCGTATGC1429940.20048646787846988TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATGGCATCTCGTATGC1426140.19995368428059992TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC1400540.1963644053054759TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCACCTCGTATGC1335170.18719912536001274TruSeq Adapter, Index 12 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC1303900.18281487717438274TruSeq Adapter, Index 12 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1156420.16213726532862927No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGTATCTCGTATGC1075360.150772149948803TruSeq Adapter, Index 12 (97% over 37bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC782130.10965948300053682TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC767950.10767135894322205TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)