FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69174733
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC46833826.770365127394131TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2413190.3488542557872974No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1596900.23085018629562332No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCAACAATCTCGTATGC906550.13105218635249377TruSeq Adapter, Index 23 (97% over 36bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT872560.12613854252245543No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCAACAATCTCGTATGC843760.12197517263998692TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAGCTCGTATGC826780.11952051914678154TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCAACAATCTCGTATGC820010.11854183810149292TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAACTCGTATGC805790.1164861742220241TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCCAACAATCTCGTATGC769130.11118655130913191TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCCAACAATCTCGTATGC766430.11079623538265049TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCCAACAATCTCGTATGC766150.11075575817545982TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCCAACAATCTCGTATGC724060.10467116656597719TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACAATCTCGTATGC711940.10291908174043837TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAACAATCTCGTATGC711730.10288872383504538TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)