FastQCFastQC Report
Sun 14 May 2023
EGAF00004457738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88540958
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATATCTCGTATGC18563032.0965472273295256TruSeq Adapter, Index 7 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4026720.45478613411885604No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3293520.3719770007458017No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGAATACCTCGTATGC1439260.1625530186831726TruSeq Adapter, Index 7 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1378380.15567710482644653No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1289960.14569076607461148No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)