FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72269279
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAATCTCGTATGC48320926.686232472306801TruSeq Adapter, Index 7 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4024930.5569351259198255No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2960100.4095931273923461No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1426330.19736325306358737No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1185250.164004680328968No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAAACTCGTATGC902190.12483727698459535TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCAATCTCGTATGC848580.11741918720401237TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCACTCAATCTCGTATGC784090.10849561678898166TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCACTCAATCTCGTATGC781700.10816490918637779TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCACTCAATCTCGTATGC777110.10752978454372016TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATTCAATCTCGTATGC766320.10603675733363827TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACTCAACCTCGTATGC749000.10364016500012405TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCACTCAATCTCGTATGC747200.10339109651280733TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCACTCAATCTCGTATGC740190.10242111312609055TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCACTCAATCTCGTATGC731380.10120206125205705TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)