FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70943118
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC879688412.399911715185679TruSeq Adapter, Index 16 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCCGT12067751.7010458998996916TruSeq Adapter, Index 16 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCC2544030.3586013797701984TruSeq Adapter, Index 16 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2237720.3154245349069659No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAAGCTCGTATGC1747680.24634947677377247TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCGCTAATCTCGTATGC1574250.2219031308998852TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCGCTAATCTCGTATGC1569330.22120961754176072TruSeq Adapter, Index 6 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1545040.21778574773102022No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCGCTAATCTCGTATGC1544380.2176927154512718TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCGCTAATCTCGTATGC1494640.21068146455023307TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAAACTCGTATGC1489140.2099061955523297TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC1436640.20250590057234305TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCGCTAATCTCGTATGC1433490.20206188287354385TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCGCTAATCTCGTATGC1375620.1939046434356043TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC1366330.19259514361914568TruSeq Adapter, Index 18 (97% over 37bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCCGTCT1218090.1716995297556558TruSeq Adapter, Index 16 (96% over 31bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC1213760.17108918161730644TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAACCTCGTATGC1105540.1558347068985606TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAATCTCGTATGC1019980.14377434045117668TruSeq Adapter, Index 16 (97% over 37bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT976930.13770609856758761No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA709960.1000745414093584No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)