FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57334804
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC28955505.050248362233871TruSeq Adapter, Index 7 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3492830.6091989082233542No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2315210.40380533959791687No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1237100.21576772112101403No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1151580.20085182466133486No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAGCTCGTATGC803540.14014873060349173TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGAACTCGTATGC724020.12627931892816796TruSeq Adapter, Index 7 (97% over 37bp)
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA625820.10915185129088432No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC596670.10406767938022427TruSeq Adapter, Index 7 (97% over 35bp)
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT591450.10315723761783506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)