FastQCFastQC Report
Sat 13 May 2023
EGAF00004457747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87494278
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC31384233.58700371240277TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2384820.27256868157709696No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1900320.2171936317938414No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAAACTCGTATGC1243800.142157867740791TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAACCTCGTATGC939270.10735216307516703TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAAGCTCGTATGC879290.10049685763450725TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)