FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68936360
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGC729141210.577019152157149TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGT13996732.0303842558556906RNA PCR Primer, Index 44 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCC4790860.6949685187903741TruSeq Adapter, Index 9 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACTATAATATCTCGTATGCCGTCT2684850.3894679092426696RNA PCR Primer, Index 44 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATAGCTCGTATGC1133220.1643863992818884TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTATAATATCTCGTATGC1104750.16025650324444168TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTATAATATCTCGTATGC1071390.1554172573080447TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTATAATATCTCGTATGC1058270.15351405267118834TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATAACTCGTATGC1052820.15272346842798198TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTATAATATCTCGTATGC1006740.1460390423863401TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTATAATATCTCGTATGC915040.13273691851440952TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTATAATATCTCGTATGC894760.12979507476170776TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATAATACCTCGTATGC797410.1156733543807651TruSeq Adapter, Index 9 (97% over 36bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)