FastQCFastQC Report
Mon 3 Apr 2023
EGAF00004457752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84376861
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC10966761.299735480797277TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4759120.5640314114079215No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3296410.39067701274168043No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1703160.2018515479024516No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1501150.17791015003509078No Hit
CGGTTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTTATCGTGTTAGTT1044990.12384793503991574No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT967770.11469613689468727No Hit
CGGGTTTATGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA844260.1000582375303106No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)