FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004457755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78949530
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC1791504722.69177156596119TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAACTCGTATGC4399020.5571939440298125TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAAGCTCGTATGC3678460.46592550962621315TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAACCTCGTATGC3239070.4102709667809295TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTCAGAATCTCGTATGC3194180.40458505579450565TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC3068190.38862675939932767TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTCAGAATCTCGTATGC2852370.3612903078713705TruSeq Adapter, Index 12 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC2788540.3532053959029269TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCAGAATCTCGTATGC2746540.34788554156053875TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTCAGAATCTCGTATGC2618400.3316549192883099TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTCAGAATCTCGTATGC2567540.3252128290060751TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTCAGAATCTCGTATGC2494800.31599934793785345TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC2426200.3073102525119529TruSeq Adapter, Index 12 (97% over 35bp)
GAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGCCGTCT2331490.2953139809698677RNA PCR Primer, Index 40 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTCAGAATCTCGTATGC2327740.2948389939750116TruSeq Adapter, Index 12 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2020230.25588879376482676No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTAGAATCTCGTATGC1800000.2279937575309188TruSeq Adapter, Index 22 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1489510.18866610098882158No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC1372240.17381230768568223TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGATCTCGTATGCC1339630.16968182077841373TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGC1265210.16025554553649654TruSeq Adapter, Index 12 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCAGAATCTCGTATGCC1194750.15133085656114736TruSeq Adapter, Index 1 (97% over 36bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT803810.10181314568940436No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)