FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78342166
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC54618486.971785794127776TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3309060.4223855643715544No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2438920.3113163861208535No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAACTCGTATGC1776750.22679357627155725TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGAGCTCGTATGC1719950.21954332996103276TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCGACCTCGTATGC1640360.20938404996359178TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1113210.14209589252357407No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1078830.1377074511828024No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCAGGCGATCTCGTATGC1045410.13344154921629303TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGTGATCTCGTATGC966560.12337672665317934TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCAGGCGATCTCGTATGC948760.12110464242206424TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCAGGCGATCTCGTATGC941950.12023537873589046TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCAGGCGATCTCGTATGC918490.11724082277735337TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGCGATCTCGTATGC900680.11496746209442307TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCAGGCGATCTCGTATGC899510.11481811723204077TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCAGGCGATCTCGTATGC875820.11179420288175337TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCAGGCGATCTCGTATGC866090.11055221526553147TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)