FastQCFastQC Report
Sat 13 May 2023
EGAF00004457757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80962915
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC20234732.4992590743552157TruSeq Adapter, Index 16 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA3287270.4060216952415807No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2756180.3404249958144417No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAACCTCGTATGC1367080.1688526160403192TruSeq Adapter, Index 16 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1190830.14708338996934583No Hit
CGGAATAGAATGGAATGGAATGGAATGGAACGGAATGGAATGGAATGGAA915160.11303446769425236No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT911360.11256511700449522No Hit
TGGAGTAGTAAGTTATAATATGGGAGATTATTTTGAAGTTTGGTAGGATA885780.10940564578239308No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)