FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76321275
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGC47885526.274203359417148TruSeq Adapter, Index 12 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4868900.6379479378456401No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3062940.4013219118784376No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1813480.23761133445425278No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1293500.16948092127653788No Hit
CGGGCGCGGTGGCGGGCGTTTGTAGTTTTAGTTATTCGGGAGGTTGAGGT1092970.14320646503874573No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCACCTCGTATGC1065600.139620309016064TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCAACTCGTATGC982550.12873867738713746TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCATGGCATCTCGTATGC803580.10528912154572889TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCAGCTCGTATGC777190.10183137008651913TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCATGGCATCTCGTATGC774740.10151035867783918TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCATCTCGTATGCC767300.10053553219597025TruSeq Adapter, Index 12 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCATGGCATCTCGTATGC763870.10008611622381831TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)