FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004457759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71834522
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC57352667.983996886622284TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAACTCGTATGC1279560.178126054767929TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCCAACAATCTCGTATGC1213240.16889372494188795TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCCAACAATCTCGTATGC1145460.15945815021919404TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCCAACAATCTCGTATGC1091400.15193252068970406TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCCAACAATCTCGTATGC1059190.1474486041683412TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCCAACAATCTCGTATGC1048890.14601475318510507TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCCAACAATCTCGTATGC1044950.1454662703818089TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCCAACAATCTCGTATGC1043420.14525328086682335TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCAACAATCTCGTATGC1031020.14352709133360697TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACAATCTCGTATGC1000810.13932159247889198TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAAGCTCGTATGC914000.12723687365804426TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCCAACAATCTCGTATGC884520.12313299725165569TruSeq Adapter, Index 23 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAACAACCTCGTATGC822430.11448952079057477TruSeq Adapter, Index 2 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA781860.10884181842262415No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAATAATCTCGTATGC777200.10819310525933479TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)