FastQCFastQC Report
Sat 13 May 2023
EGAF00004457761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92708594
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC90640059.776876780161286TruSeq Adapter, Index 3 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCC3477170.3750644735265859TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1930190.2081996842709102No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGTTATCTCGTATGC1792180.19331325421675577TruSeq Adapter, Index 12 (97% over 38bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC1726030.18617799337998805TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGCTATCTCGTATGC1666820.1797913147081057TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCTAGCTATCTCGTATGC1658910.1789381036239208TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCTAGCTATCTCGTATGC1594300.1719689546796492TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCTAGCTATCTCGTATGC1555450.1677784046644047TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCTAGCTATCTCGTATGC1487680.1604684027459202TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCTAGCTATCTCGTATGC1481640.15981689895976633TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCTAGCTATCTCGTATGC1480450.1596885397701102TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAGCTCGTATGC1340690.1446133462017556TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1326330.14306440673666135No Hit
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC1266400.13660006536179375TruSeq Adapter, Index 12 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGC1225560.13219486426468727TruSeq Adapter, Index 12 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTAACTCGTATGC1223710.13199531426396133TruSeq Adapter, Index 3 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTATCTCGTATGCCGT1003270.10821758336665098TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAGCTACCTCGTATGC993070.10711736174102696TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)