FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80943410
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC1125439213.904025046634432TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGCGCTAATCTCGTATGC2236210.2762683212876749TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC2167310.26775620152400303TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGCTAATCTCGTATGC2099050.25932314934594425TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGCGCTAATCTCGTATGC2023650.2500079994158882TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGCGCTAATCTCGTATGC1960470.24220254619863432TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGCGCTAATCTCGTATGC1860070.22979881870556232TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAAACTCGTATGC1858970.22966292129278965TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGCGCTAATCTCGTATGC1853420.2289772570738001TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGCGCTAATCTCGTATGC1807970.2233622230642371TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAAGCTCGTATGC1771040.21879977628814995TruSeq Adapter, Index 16 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCC1761090.21757052241806962TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGTTAATCTCGTATGC1640870.20271817063303854TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC1545330.19091486261821686TruSeq Adapter, Index 6 (97% over 35bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC1523670.18823891901761985TruSeq Adapter, Index 6 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAACCTCGTATGC1253380.1548464538373167TruSeq Adapter, Index 16 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1085650.13412456925153016No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTATCTCGTATGCC919010.11353734664749113TruSeq Adapter, Index 16 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)