FastQCFastQC Report
Sat 13 May 2023
EGAF00004457763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87181283
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGC24011352.7541863544265572TruSeq Adapter, Index 16 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2420650.27765707462690126No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1722670.19759631204326278No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTAATCTCGTATGCC1249940.1433725172408853TruSeq Adapter, Index 16 (97% over 35bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT915900.10505695356651268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)