FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004457764

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457764
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83619626
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC48880395.845564293722147TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2949810.35276527067939767No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA2119260.25344050211370234No Hit
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1075700.12864204869799345No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTAATCGATCTCGTATGC1015820.12148105039360019TruSeq Adapter, Index 10 (97% over 35bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA997060.11923755793885038No Hit
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAATCGATCTCGTATGC940230.112441306542079TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATTGATCTCGTATGC904950.10822220132866893TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAGCTCGTATGC890040.10643912710157302TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGAACTCGTATGC883880.10570245793732683TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTAATCGATCTCGTATGC876070.10476846667551466TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTAATCGATCTCGTATGC873870.10450537054542675TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTAATCGATCTCGTATGC869760.10401385913876247TruSeq Adapter, Index 10 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)