FastQCFastQC Report
Sun 2 Apr 2023
EGAF00004457767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72243642
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC59587198.248087769439975TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAACTCGTATGC3545880.49082243112826457TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGACCTCGTATGC2939710.40691608543212704TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA2529300.35010693397766407No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGAGCTCGTATGC2499900.34603737170393484TruSeq Adapter, Index 3 (97% over 37bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA1909540.2643194538835681No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC1098630.15207289798595702TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATGTAGAGATCTCGTATGC1047740.14502867947881143TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGTAGAGATCTCGTATGC1029230.14246651629218804TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGTAGAGATCTCGTATGC1022370.14151695176165122TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGTAGAGATCTCGTATGC970830.13438275993892998TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGTAGAGATCTCGTATGC952310.1318192125474516TruSeq Adapter, Index 18 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACGTAGAGATCTCGTATGC930160.12875319879360456TruSeq Adapter, Index 18 (97% over 35bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT883810.1223374092906335No Hit
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA856630.11857514049471649No Hit
GATCGGAAGAGCATACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC772070.10687030424075242TruSeq Adapter, Index 18 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)