FastQCFastQC Report
Wed 22 Feb 2023
EGAF00004457768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70865792
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC61971988.744978112994207TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACGCGTCTGAACTCCAGTCACTCATTCATCTCGTATGC2675990.3776137857882122TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCATTCATCTCGTATGC1221440.17235960616936308TruSeq Adapter, Index 3 (97% over 34bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC1196420.16882898874537378TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCATTCATCTCGTATGC1133230.1599121336285919TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCATTCATCTCGTATGC1131260.1596341433677902TruSeq Adapter, Index 3 (97% over 34bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA1113490.15712658654827424No Hit
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTCATTCATCTCGTATGC1070800.15110252348552033TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTCATTCATCTCGTATGC1063870.1501246186594514TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCATTCATCTCGTATGC1063810.15011615195100056TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTATTCATCTCGTATGC948630.1338628939615887TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTCATTCATCTCGTATGC933770.1317659725019372TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTCATTCATCTCGTATGC883790.12471320436240943TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAGCTCGTATGC871060.12291685105276182TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACCGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC870460.12283218396825367RNA PCR Primer, Index 45 (100% over 31bp)
GATCGGAAGAGCACTCGTCTGAACTCCAGTCACTCATTCATCTCGTATGC841080.1186863190635053TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCAACTCGTATGC838630.11834059513509707TruSeq Adapter, Index 3 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA789110.11135273842702556No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTTATCTCGTATGC734990.10371576740439167TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCACCTCGTATGC731230.10318518700814068TruSeq Adapter, Index 3 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCCGTCT721400.10179805794028238RNA PCR Primer, Index 45 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATTCATCTCGTATGCC718320.10136343357314062TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)