FastQCFastQC Report
Tue 26 Sep 2023
EGAF00004457769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004457769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90170134
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC1138745212.628851144881297TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAACCTCGTATGC13612201.509612927934653TruSeq Adapter, Index 10 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAAACTCGTATGC5292330.5869271526201791TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTCGAGTAGTTGGGATTATA4755130.5273508853829584No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAAGCTCGTATGC3278330.3635716012133241TruSeq Adapter, Index 10 (97% over 36bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATA3214290.35646947136620644No Hit
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC2210840.24518539586510985TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTCGGCAATCTCGTATGC1951710.21644749912426656TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTCGGCAATCTCGTATGC1897150.21039671516957045TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTCGGCAATCTCGTATGC1824440.20233306961704192TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATTCGGCAATCTCGTATGC1793300.19887959798307497TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTCGGCAATCTCGTATGC1787860.19827629401105248TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGGCAATCTCGTATGC1665340.18468864646469305TruSeq Adapter, Index 20 (97% over 38bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGT1645070.18244067376011663No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTCGGCAATCTCGTATGC1632360.18103111613430672TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC1504570.16685901786505053TruSeq Adapter, Index 3 (97% over 34bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC1502840.16666715832983015TruSeq Adapter, Index 3 (97% over 34bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTGAGTAGTTGGGATTATA1416130.1570508922610673No Hit
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTCGGCAATCTCGTATGC997260.11059759542999016TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGGTAATCTCGTATGC972120.10780953258869505TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)